Physicochemical Properties of Drugs (Undergraduate) and Analytical Mass Spectrometry (Graduate)
- Analytical mass spectrometry
- The biological fate of lipid based drug delivery systems
- Targeted metabolomics
- Substance abuse
Basic Science: Three main streams are being evaluated, focusing on the use of mass spectrometry (MS) for the analysis of analytes in both qualitative and quantitative applications.
Stream 1: MS Analysis of lipid-based drug delivery agents: The goal is to establish fast, robust and sensitive analytical methods that will allow for the determination of the biological fate of lipid-based drug delivery agents. We have established the tandem mass spectrometric (MS/MS) fingerprints of over 50 gemini surfactants, used as drug delivery agents. Six different MS-based quantification strategies (within tissue culture lysate) were developed and validated. Various separation strategies, ionization methods (i.e. ESI, MALDI, DESI) and mass analyzers (i.e. quadrupole, orboitrap, ion trap and ToF) were used and compared to evaluate the quantitative capabilities of each strategy.
We are currently assessing the cellular uptake and removal of gemini surfactants as well as their sub-cellular distribution. Initial data showed that varying structures will have similar uptake profiles with different subcellular distribution. The latter may provide insights into their efficiency and toxicity. In addition, we will develop MS-based methods to determine tissue distribution of novel gemini surfactant formulations. The goal is to highlight the relationship between the structure, efficiency and toxicity, so that newer and safer compounds are synthesized.
Stream 2: Urine metabolomics for biomarker discovery in respiratory illness: Asthma and chronic obstructive pulmonary disease (COPD) are common respiratory illnesses that reduce the quality of life and can result in premature death. Asthma, in particular, is common among young children, a vulnerable population that cannot perform conventional clinical diagnostic tests.
In collaboration with Dr. Darryl Adamko, College of Medicine, University of Saskatchewan, we have been developing targeted HPLC-MS/MS methods for the analysis of 50 urinary metabolites that are linked to respiratory illness. Preliminary data showed separation among various test subjects based on their clinical condition. The end goal is to develop clinical MS-based testing that can aid in disease diagnosis and management.
Stream 3: Extraction of active metabolites from canola waste stream: Canada is a major world producer of canola oil. By-products from the crushing and refining process are either treated as waste or low value products. Therefore, extracting high value compounds from canola by-products represents an important value-added opportunity. We are currently developing extraction strategies to isolate biologically active metabolites from canola’s waste stream. In addition, LC-MS/MS methods are being developed to profile the various extracts. Nanotechnology will be employed to formulate the isolated metabolites into functional foods or tested for therapeutic benefits.
Applied Health Research: We evaluated barriers to accessing health services by People who Inject Drugs (PWIDs) in the city of Saskatoon, Canada. Focus groups with PWIDs and service providers revealed that stigma/discrimination, gaps in education/training and lack of resources hindered PWIDs access to services. Subsequently, we evaluated the educational needs (knowledge and skills) of pharmacists in the area of substance abuse. Based on the findings, we aim to develop and evaluate educational workshops [focusing on addiction management] for pharmacists so that appropriate care is provided to patients struggling with the disease of addiction.
- Chitanda JM, Zhang H, Pahl E, Purves RW and El-Aneed A. The Development of Novel Nanodiamond Based MALDI Matrices for the Analysis of Small Organic Pharmaceuticals. Journal of the American Society for Mass Spectrometry. 27, 2016:1686-93.
- El-Aneed A. Is HPLC-MS/MS the “Gold Standard” for Quantitative Analysis? J Pharm Anal Insights 1(4), 2016: doi http://dx.doi.org/10.16966/ jpai.e104
- Willems J, Khamis M.M, Mohammed-Saeid W, Purves RW, Katselis G, Low N, El-Aneed A. Analysis of a Series of Chlorogenic Acid Isomers using Differential Ion Mobility and Tandem Mass Spectrometry. Analytica Chimica Acta. 933, 2016: 164-174.
- Donkuru M, Michel D, Awad H; Katselis G; El-Aneed A. Hydrophilic interaction liquid chromatography- tandem mass spectrometry quantitative method for the cellular analysis of varying structures of gemini surfactants designed as nanomaterial drug carriers. Journal of Chromatography A. 1446, 2016: 114–124.
- Awad H, Das U, Dimmock J, El-Aneed A. Establishment of Tandem Mass Spectrometric Fingerprint of Novel Antineoplastic Curcumin Analogues using Electrospray Ionization. Rapid Communications in Mass Spectrometry. 29, 2015: 1307-1316.
- Michel D, Gaunt M, Arnason T, and El-AneedA. Development and validation of fast and simple flow injection analysis-tandem mass spectrometry (FIA-MS/MS) for the determination of metformin in dog serum. Journal of Pharmaceutical and Biomedical Analysis. 107, 2015: 229-235.
- Awad H, Khamis M and El-Aneed A. Mass spectrometry, review of the basics: ionization. Applied Spectroscopy Reviews. 50 (2) 2015: 158-175.
- Khamis M, Adamko D, and El-Aneed A. Mass Spectrometric Based Approaches in Urine Metabolomics and Biomarker Discovery. Mass Spectrometry Reviews. 9999, 2014, 1-20. DOI: 10.1002/mas.21455
- Buse J, Purves RW, Verrall RE, Badea I, Zhang H, Mulligan CC, Peru KM, Bailey J, Headley JV, El-Aneed A. The Development and assessment of high-throughput mass spectrometry-based methods for the quantification of a nanoparticle drug delivery agent in cellular lysate. Journal of Mass Spectrometry. 49 (11), 2014: 1171-1180.
- Awad H, Stoudemayer MJ, Usher L, Amster J, Cohen A, Das U, R. Whittal M, Dimmock J, and El-Aneed A.The unexpected formation of [M-H]+ species during MALDI and Dopant free-APPI MS analysis of novel antineoplastic curcumin analogues. Journal of Mass Spectrometry. 49 (11), 2014: 1139-1147.
- Donkuru M, Chitanda JM, Verrall RE and El-Aneed A. Multi-stage tandem mass spectrometric analysis of novel β-cyclodextrin-substituted and novel bis-pyridinium gemini surfactants designed as nanomedical drug delivery agents. Rapid Communications in Mass Spectrometry. 28 (7), 2014. 28: 757-772.
- Awad H and El-Aneed A. Enantioselectivity of Mass Spectrometry: Challenges and Promises. Mass Spectrometry Reviews. 32 (6), 2013: 466–483.
- Lang K, Neil J, Wright J, Dell CA, Berenbaum S, and El-Aneed A. Qualitative investigation of barriers to accessing care by people who inject drugs in Saskatoon, Canada: perspectives of service providers. Substance Abuse Treatment, Prevention, and Policy. 2013. 8 :35 (1 October 2013)
- Buse J, Badea I, Verrall RE, and El-Aneed A. A general liquid chromatography tandem mass spectrometry method for the quantitative determination of diquaternary ammonium gemini surfactant drug delivery agents in mouse keratinocytes' cellular lysate. Journal of Chromatography A. 1294, 2013: 98–105.
- Lang K, El-Aneed A, Berenbaum S, Dell CA, Wright J, Teed McKay Z. Qualitative Assessment of Crisis Services among Persons using Injection Drugs in the City of Saskatoon. Journal of Substance Use. 18, (1) 2013: 3-11.
- Mohammed-Saeid W, Michel D, El-Aneed A, Verrall R, Low N, and Badea A. Development of lyophilized gemini surfactant-based lipoplexes: influence of lyophilization on the Structure, Activity and Stability of the Lipoplexes. Journal of Pharmacy & Pharmaceutical Sciences 15, 2012: 548-567.
- Michel, D, Chitanda, J, Balogh R, Singh J, Das U, El-Aneed A, Dimmock J, Verrall R, and Badea, I. Design and evaluation of cyclodextrin-based delivery systems to incorporate poorly soluble curcumin analogs for the treatment of melanoma. Eur J Pharm Biopharm. 81, 2012: 548-556.
- Mohammed-Saeid W, Buse J, Badea I, Verrall RE, and El-Aneed A. Mass spectrometric analysis of amino acid/di-peptide modified gemini surfactants used as gene delivery agents: Establishment of a universal mass spectrometric fingerprint. International Journal of Mass Spectrometry. 309, 2012: 182-191.