AB SCIEX 4000 QTRAP® and AB SCIEX 6500 QTRAP® with Ion Mobility

The QTRAP® based LC/MS/MS Systems (4000 and 6500) are hybrid triple quadrupole/linear ion trap mass spectrometers coupled to high-pressure liquid chromatography. The systems have high-sensitivity and are ideal for quantification of small molecules in complex biological, agricultural and environmental matrices. The LC/MS/MS systems provide specificity of an analyte in complex matrices, such as blood, that conventional HPLC methods cannot achieve. In addition to targeted quantification analysis, the systems can perform MS experiments (such as MS3 and precursor ion scan) that aid in structural characterization and identification of unknown compounds. Finally, the 6500 system is equipped with SelexION®, a Differential Ion Mobility Spectrometry interface, capable of separating isomeric species before their introduction to the MS. In addition, ultra high-performance liquid chromatography (UHLPC) is available for those interested in faster run times.

We also have triple quadruple (QqQ) Thermo TSQ Altis equipped with HPLC and Ion Chromatography (IC) for specialized analysis.

Our current workflows include targeted quantitative analysis of small organic compounds as well as screening/profiling experiments. Compounds that include metabolites, pharmaceuticals, lipids, pesticides, toxins, hormones, peptides, drug delivery agents, and therapeutic agents. In addition to quantification, screening/profiling experiments can be developed to target a specific class of compounds that share structural similarities.

High Pressure Liquid Chromatography

Two Agilent 1200 HPLC systems, equipped with UV-VIS and fluorescence detection, are available in the hub. One system also has a fraction collector to isolate compounds as needed. A third system is a Waters Alliance 2695 equipped with UV-VIS detection. The systems can be used when the selectivity and sensitivity of LC-MS is not needed.